Application

Research CategoryApoptosis & Cancer

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Del-1 in human cerebral cortex tissue sections.

ELISA Analysis: A representative lot detected Del-1 in ELISA applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72; Maekawa, T., et. al. (2015). Nat Commun. 6:8272).

Inhibition Analysis: A representative lot inhibited the uptake of BODIPY-labeled platelet microparticles by endothelium. (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).

Western Blotting Analysis: A representative lot detected Del-1 in Western Blotting applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).

Flow Cytometry Analysis: A representative lot detected Del-1 in Flow Cytometry applications (Dasgupta, S.K., et. al. (2012). Ciculation. 125(13):1664-72).

Detects Del-1 using this Anti-Del-1, clone D345, Cat. No. MABS1976, is a mouse monoclonal antibody that detects Developmentally-regulated endothelial cell locus 1 protein (Del-1) and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assay, & Western Blot.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

EGF-like repeat and discoidin I-like domain-containing protein 3 (UniProt: O43854; also known as Developmentally-regulated endothelial cell locus 1 protein, Integrin-binding protein DEL1, Del-1) is encoded by the EDIL3 (also known as DEL1) gene (Gene ID: 10085) in human. Del-1 is a calcium-dependent glycoprotein that is secreted by endothelial cells and is shown to be expressed in a developmentally restricted pattern during embryogenesis. In adult tissues it is expressed in endothelial cells and in macrophages. Del-1 is reported to prevent apoptosis in endothelial cell subjected to TNF alpha, etoposide, and anoikis treatment. This anti-apoptotic effect is shown to be mediated specifically through binding of integrin alphav beta3 by the RGD motif. Del-1 expression is reported to be inversely related to that of IL-17 that is shown to inhibit Del-1 via a GSK-3beta and C/EBPb-dependent mechanism. This inhibitory action can be reversed at the PI3K/Akt signaling induced by D-resolvins. It serves as an endothelial cell-secreted homeostatic factor that regulates local inflammation in several tissues. It is reported to interfere with beta2-integrin-dependent inflammatory cell adhesion to endothelium and restricts transendothelial migration of neutrophils. Del-1 is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off in the mature form. It has three EGF-like domains in its N-terminal region. EGF-like domains 2 and 3 form a rigid rod via an interdomain calcium ion binding site, while the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Del-1 also has discoidin I-like domains at the C-terminal region that mediate its binding to phosphatidylserine. Two isoforms of Del-1 have been described that are produced by alternative splicing. (Ref.: Dasgupta, SK et al. (2012). Circulation 125 (13); 1664-1672; Wang, Z., et al. (2012). Surgery 151(2); 296-305; Maekawa, T et al. (2015). Nat. Commun. DOI: 10.1038/ncomms9272).

Immunogen

GST-tagged full length human recombinant Developmentally-regulated endothelial cell locus 1 protein (Del-1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Del-1 in human pancreas tissue sections.

Specificity

Clone D345 detects human Developmentally-regulated endothelial cell locus 1 protein (Del-1).

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

53.76 kDa calculated.